Environmental (e)RNA advances the reliability of eDNA by predicting its age

Scientific report: eRNA publication from NOAA (National Oceanic and Atmospheric Administration) GLERL (The Great Lakes Environmental Research Laboratory)


Published: 02 February 2021

Environmental (e)RNA advances the reliability of eDNA by predicting its age

Scientific Reports volume 11, Article number: 2769 (2021)

Abstract

Environmental DNA (eDNA) analysis has advanced conservation biology and biodiversity management. However, accurate estimation of age and origin of eDNA is complicated by particle transport and the presence of legacy genetic material, which can obscure accurate interpretation of eDNA detection and quantification. To understand the state of genomic material within the environment, we investigated the degradation relationships between (a) size of fragments (long vs short), (b) genomic origins (mitochondrial vs nuclear), (c) nucleic acids (eDNA vs eRNA), and (d) RNA types (messenger (m)RNA vs ribosomal (r)RNA) from non-indigenous Dreissena mussels. Initial concentrations of eRNA followed expected transcriptional trends, with rRNAs found at > 1000 × that of eDNA, and a mitosis-associated mRNA falling below detection limits within 24 h. Furthermore, the ratio of eRNA:eDNA significantly decreased throughout degradation, potentially providing an estimate for the age of genomic material. Thus, eRNA quantification can increase detection due to the high concentrations of rRNAs. Furthermore, it may improve interpretation of positive detections through the eRNA:eDNA ratio and/or by detecting low abundant mitosis-associated mRNAs that degrade within ~ 24 h.


Scientific Reports volume 11, Article number: 2769 (2021)

Schematic of experimental design, in which the degradation rates of environmental (e)DNA and (e)RNA were compared across multiple genes collected in environmental genomic material released from dreissenid mussels. The concentration of eRNA depletes faster than eDNA, providing a predictor for estimating time since genomic material release.


To read the full article visit the Nature.com website at: https://www.nature.com/articles/s41598-021-82205-4

7 views0 comments
4deeb4_8c12ff1bf81e4c4c9d551e2c15285c3e~

We envision a world in which whole aquatic environmental management is possible for public health, conservation, commerce, and recreation.

Subscribe 

info@eqo.life

 

6101 Highland Campus Dr.

Austin, TX 78752

  • Twitter
  • Facebook
  • LinkedIn

Copyright © 2020 EQO Inc. Created by Push Creative Designs